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生物活性
Nocodazole is a reversible anti-neoplastic agent which exerts its effect in cells by interfering with the polymerization of microtubules (tubulin). Inhibits autophagosome-lysosome fusion. Enhances homology-directed repair (HDR) efficiency 9 to 31% (depending on cell cycle phase) and increases Cas9-mediated gene editing frequencies.
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体外研究
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体内研究
1% DMSO+30% polyethylene glycol+1% Tween 80
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激酶实验
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细胞实验
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动物实验
5 mg/kg 腹腔注射
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不同实验动物依据体表面积的等效剂量转换表（数据来源于FDA指南）
|  动物 A (mg/kg) = 动物 B (mg/kg)×动物 B的Km系数/动物 A的Km系数 |
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例如，已知某工具药用于小鼠的剂量为88 mg/kg , 则用于大鼠的剂量换算方法：将88 mg/kg 乘以小鼠的Km系数（3），再除以大鼠的Km系数（6），得到该药物用于大鼠的等效剂量44 mg/kg。
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参考文献
[1] Park H, et al. ChemMedChem. 2012, 7(1), 53-56.
[2] Hoebeke, J., et al. 1976. Biochem. Biophys. Res. Commun. 69: 319-324.
[3] Lin et al (2014) Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery. Elife 3 e04766.
[4] Mizushima et al (2010) Methods in mammalian autophagy research. Cell 140 313.
[5] Webb et al (2004) Microtubule disruption inhibits autophagosome-lysosome fusion: implications for studying the roles of aggresomes in polyglutamine diseases. Int.J.Biochem.Cell Biol. 36 2541.
[6] Vasquez et al (1997) Nanomolar concentrations of nocodazole alter microtubule dynamic instability in vivo and in vitro. Mol.Biol.Cell 8 973.
分子式
C14H11N3O3S |
分子量
301.32 |
CAS号
31430-18-9 |
储存方式
﹣20 ℃冷藏长期储存。冰袋运输 |
溶剂（常温）
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DMSO
10 mg/mL |
Water
<1 mg/mL |
Ethanol
<1 mg/mL |
体内溶解度
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Clinical Trial Information ( data from http://clinicaltrials.gov )
注：以上所有数据均来自公开文献，并不保证对所有实验均有效，数据仅供参考。
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