生物活性
PtdIns (4)-P1-fluorescein (ammonium salt) is a phosphatidylinositol derivative conjugated with fluorescein, and it serves as an important tool for studying phosphatidylinositol metabolism and cellular signaling pathways. As a fluorescently labeled phosphatidylinositol 4-phosphate (PtdIns(4)P), it allows researchers to observe and quantify the spatial distribution of phosphatidylinositol in living or fixed cells using fluorescence microscopy or flow cytometry. This fluorescent probe facilitates tracking the dynamic changes of PtdIns(4)P within cells, where PtdIns(4)P is fundamental to regulating vesicle trafficking, cytoskeletal organization, and signal transduction.
PtdIns(4)P can specifically interact with proteins containing PtdIns(4)P-binding domains (such as FYVE and PH domains), thereby clarifying their spatial relationships in living cell systems. The fluorescein tag enables easy detection while preserving the native functions of the phospholipid, aiding in gaining insights into the dynamics of PtdIns(4)P, which is primarily localized to the Golgi apparatus, endosomes, and plasma membrane.
Furthermore, this labeled compound can be used to screen potential inhibitors or activators, such as phosphocreatine kinases and phosphatases that regulate phosphocreatine metabolism. Therefore, PtdIns (4)-P1-fluorescein contributes to mapping the regulatory network of phosphocreatine signal transduction, thereby advancing research on lipid signaling pathways and membrane transport processes.
