AMQUAR蛋白酶抑制剂Cocktail (EDTA-Free)包含有六种广谱抑制剂,能够抑制天冬氨酸蛋白酶,半胱氨酸蛋白酶,丝氨酸蛋白酶以及氨肽酶等的活性。在蛋白提取及纯化的实验中,它能阻止蛋白被内源性蛋白酶体降解。
1. AMQUAR蛋白酶抑制剂Cocktail (EDTA-Free)包含有六种广谱抑制剂,能够抑制天冬氨酸蛋白酶,半胱氨酸蛋白酶,丝氨酸蛋白酶以及氨肽酶等的活性。在蛋白提取及纯化的实验中,它能阻止蛋白被内源性蛋白酶体降解。 2. AMQUAR蛋白酶抑制剂Cocktail主要用于哺乳动物细胞及组织的提取液中,同时也适用于植物,细菌的提取液中。 3. AMQUAR蛋白酶抑制剂Cocktail贮存液浓度为100X in DMSO, 使用时通常按照1:100进行稀释。 The protease inhibitor cocktail prevented the A549 cell detachment induced by meconium. It shows significant protective effects, increasing the number of protected cells by 135%.[1] The protease cocktail has no effect on oral bacterial growth or total bacterial composition. It means that the addition of protease inhibitors in the preparation of saliva samples for protein research will not interfere with microbial DNA analysis.[2] PIC completely blocked NP-dependent degradation of thymic stromal lymphopoietin (TSLP), suggesting that TSLP was truncated by endogenous tissue proteases.[3] A protease inhibitor cocktailcan increase the yield of recombinant mouse hepcidin-1 to up to 25μg/ml in BES.[4] Metabolic turnover of127I-ACTH (4–10) was not NADPH-dependent. And, without EDTA, the protease inhibitor cocktail can only incompletely inhibit proteolysis.[5] Protease inhibitor cocktail prevent estrogen receptor (ER) enzymatic degradation by competition and involvement in ER stability.[6]
–20 °C保存,一年有效。
1. 将蛋白酶抑制剂Cocktail试管置于室温融化。
2. 涡旋混匀。
3. 按照1:100的稀释比将Cocktail加入裂解液中(如细胞裂解液或组织提取液),然后涡旋混匀。
1. AMQUAR蛋白酶抑制剂只是广谱的蛋白酶抑制剂,并不包含磷酸酶抑制剂。
2. 通常Cocktail的工作浓度为1X,但是如果一些样品的某些蛋白酶含量较高,需要对Cocktail工作浓度进行优化。
3. Cocktail在–20 °C条件下是结冻的。使用前可置于室温融化,或者将试管置于拇指与食指之间进行融化。
注:以上所有数据均来自公开文献,并不保证对所有实验均有效,数据仅供参考。